Bicistronic expression strategy for high-level expression of recombinant proteins in Corynebacterium glutamicum.

ABSTRACT: Directly using the promoter associated with 5'-untranslated region of a high-protein-abundance gene from the genome may cause low expression activity of an expression system. A bicistronic expression part containing the short 5' coding sequence of the source gene and an embedded Shine-Dalgarno sequence can cause higher expression levels of the recombinant gene in a bicistronic cassette. Here, we evaluated two methods to construct expression parts and exploited genomic sequence sources to provide specific functional sequences to complete the expression system. The architecture of the bicistronic part increased the expression levels of target genes and performed more reliably than conventional expression parts with the same promoter and 5' untranslated region. For Corynebacterium glutamic

SUBMITTER: Liu X 

PROVIDER: S-EPMC6999366 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature