Development and performance of a 3D-printable poly(ethylene glycol) diacrylate hydrogel suitable for enzyme entrapment and long-term biocatalytic applications.

ABSTRACT: Physical entrapment of enzymes within a porous matrix is a fast and gentle process to immobilize biocatalysts to enable their recycling and long-term use. This study introduces the development of a biocompatible 3D-printing material suitable for enzyme entrapment, while having good rheological and UV-hardening properties. Three different viscosity-enhancing additives have been tested in combination with a poly(ethylene glycol) diacrylate-based hydrogel system. The addition of polyxanthan or hectorite clay particles results in hydrogels that degrade over hours or days, releasing entrapped compounds. In contrast, the addition of nanometer-sized silicate particles ensures processability while preventing disintegration of the hydrogel. Lattice structures with a total height of 6 mm consisting

SUBMITTER: Schmieg B 

PROVIDER: S-EPMC6999379 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature