Evaluation of pyruvate decarboxylase-negative Saccharomyces cerevisiae strains for the production of succinic acid.

ABSTRACT: Dicarboxylic acids are important bio-based building blocks, and Saccharomyces cerevisiae is postulated to be an advantageous host for their fermentative production. Here, we engineered a pyruvate decarboxylase-negative S. cerevisiae strain for succinic acid production to exploit its promising properties, that is, lack of ethanol production and accumulation of the precursor pyruvate. The metabolic engineering steps included genomic integration of a biosynthesis pathway based on the reductive branch of the tricarboxylic acid cycle and a dicarboxylic acid transporter. Further modifications were the combined deletion of GPD1 and FUM1 and multi-copy integration of the native PYC2 gene, encoding a pyruvate carboxylase required to drain pyruvate into the synthesis pathway. The effect of increased

SUBMITTER: Zahoor A 

PROVIDER: S-EPMC6999389 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature