Enhancing extracellular protein production in Escherichia coli by deleting the d-alanyl-d-alanine carboxypeptidase gene dacC.

ABSTRACT: d-Alanyl-d-alanine carboxypeptidase DacC is important for synthesis and stabilization of the peptidoglycan layer of Escherichia coli. In this work, dacC of E. coli BL21 (DE3) was successfully deleted, and the effects of this deletion on extracellular protein production in E. coli were investigated. The extracellular activities and fluorescence value of recombinant amylase, green fluorescent protein, and α-galactosidase of the deletion mutants were increased by 82.3, 29.1, and 37.7%, respectively, compared with that of control cells. The outer membrane permeability and intracellular soluble peptidoglycan accumulation of deletion mutant were also enhanced compared with those of control cells, respectively. Based on fluorescence-assisted cell sorting

SUBMITTER: Hu J 

PROVIDER: S-EPMC6999477 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature