Genome-wide evolution of wobble base-pairing nucleotides of branchpoint motifs with increasing organismal complexity.

ABSTRACT: How have the branchpoint motifs evolved in organisms of different complexity? Here we identified and examined the consensus motifs (R₁C₂T₃R₄A₅Y₆, R: A or G, Y: C or T) of 898 fungal genomes. In Ascomycota unicellular yeasts, the G₄/A₄ ratio is mostly (98%) below 0.125 but increases sharply in multicellular species by about 40 times on average, and in the more complex Basidiomycota, it increases further by about 7 times. The global G₄ increase is consistent with A₄ to G₄ transitions in evolution. Of the G₄/A₄-interacting amino acids of the branchpoint binding protein MSL5 (SF1) and the HSH155 (SF3B1), as well as the 5' splice sites (SS) and U2

SUBMITTER: Nguyen H 

PROVIDER: S-EPMC6999648 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature