Meg3 Induces EMT and Invasion of Glioma Cells via Autophagy.

ABSTRACT:

Background

Glioma is one of the most common malignant tumors. Glioblastoma (grade IV) is considered the most malignant form of human brain tumors. Maternal expression gene 3 (Meg3) encodes a non-coding RNA (ncRNA) that plays an important role in the development and progression of cancer. However, the role of Meg3 in glioma cells remains largely unclear.

Methods

Reverse transcription-quantitative (RT-q) PCR was conducted to evaluate the mRNA expression related to cell autophagy and EMT while protein expression was detected by Western blotting. Staining of acidic vacuoles and immunofluorescence staining were used to detect autophagy. The ability of cells to migrate and invade was detected by Transwell migration and invasion assays.

Results

In the present study, it was f

SUBMITTER: Yang Z 

PROVIDER: S-EPMC6999788 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature