Thorough Performance Evaluation of 213 nm Ultraviolet Photodissociation for Top-down Proteomics.

ABSTRACT: Top-down proteomics studies intact proteoform mixtures and offers important advantages over more common bottom-up proteomics technologies, as it avoids the protein inference problem. However, achieving complete molecular characterization of investigated proteoforms using existing technologies remains a fundamental challenge for top-down proteomics. Here, we benchmark the performance of ultraviolet photodissociation (UVPD) using 213 nm photons generated by a solid-state laser applied to the study of intact proteoforms from three organisms. Notably, the described UVPD setup applies multiple laser pulses to induce ion dissociation, and this feature can be used to optimize the fragmentation outcome based on the molecular weight of the analyzed biomolecule. When applied to complex proteoform mi

SUBMITTER: Fornelli L 

PROVIDER: S-EPMC7000117 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature