Developments in zebrafish avatars as radiotherapy sensitivity reporters - towards personalized medicine.

ABSTRACT:

Background

Whereas the role of neoadjuvant radiotherapy in rectal cancer is well-established, the ability to discriminate between radioresistant and radiosensitive tumors before starting treatment is still a crucial unmet need. Here we aimed to develop an in vivo test to directly challenge living cancer cells to radiotherapy, using zebrafish xenografts.

Methods

We generated zebrafish xenografts using colorectal cancer cell lines and patient biopsies without in vitro passaging, and developed a fast radiotherapy protocol consisting of a single dose of 25 Gy. As readouts of the impact of radiotherapy we analyzed proliferation, apoptosis, tumor size and DNA damage.

Findings

By directly comparing isogenic cells that only differ in the KRAS^G13D allele, we show th

SUBMITTER: Costa B 

PROVIDER: S-EPMC7000345 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature