Contribution of synergism between PHF8 and HER2 signalling to breast cancer development and drug resistance.

ABSTRACT:

Background

HER2 plays a critical role in tumourigenesis and is associated with poor prognosis of patients with HER2-positive breast cancers. Although anti-HER2 drugs are beneficial for treating breast cancer, de novo, or acquired resistance often develops. Epigenetic factors are increasingly targeted for therapy; however, such mechanisms that interact with HER2 signalling are poorly understood.

Methods

RNA sequencing was performed to identify PHF8 targets downstream of HER2 signalling. CHIP-qPCR were used to investigate how PHF8 regulates HER2 transcription. ELISA determined cytokine secretion. Cell-based assay revealed a feed forward loop in HER2 signalling and then evaluated in vivo.

Findings

We report the synergistic interplay between histone demethylase PHF8 and H

SUBMITTER: Liu Q 

PROVIDER: S-EPMC7000350 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature