Culture dependent and independent analyses suggest a low level of sharing of endospore-forming species between mothers and their children.

ABSTRACT: Spore forming bacteria comprise a large part of the human gut microbiota. However, study of the endospores in gut microbiota is limited due to difficulties of culturing and numerous unknown germination factors. In this study we propose a new method for culture-independent characterization of endospores in stool samples. We have enriched DNA of spore-forming bacterial species from stool samples of 40 mother-child pairs from a previously described mother-child cohort. The samples were exposed to a two-step purification process comprising ethanol and ethidium monoazide (EMA) treatment to first kill vegetative cells and to subsequently eliminate their DNA from the samples. The composition of the ethanol-EMA resistant DNA was characterized by 16S rRNA marker gene sequencing. Operational taxonom

SUBMITTER: Avershina E 

PROVIDER: S-EPMC7000398 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature