Enhanced sensitivity and detection range of FRET-based vitamin B₁₂ nanosensor.

ABSTRACT: Vitamin B₁₂ (cobalamin) is a cobalt-containing compound that acts as an essential co-factor for various enzymes involved in the metabolic processes of the living cells. The constructed FRET Sensor for Vitamin Anemia Linked (SenVitAL) displayed marginal FRET efficiency. Here, we report the development of a molecular SenVitAL containing enhanced cyan fluorescent protein (ECFP) and venus as FRET pair to improve the FRET efficiency for optical imaging and screening of already developed sensor by our group. The sensor is the improved version of previously reported SenVitAL and consists of ECFP/venus as FRET pair instead of the originally used pair CFP/YFP. To increase the physiological range of vitamin B₁₂ measurement, affinity mutants were created. Compared to the wild ty

SUBMITTER: Soleja N 

PROVIDER: S-EPMC7000600 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature