Transcriptome sequencing of endometrium revealed alterations in mRNAs and lncRNAs after ovarian stimulation.

ABSTRACT:

Research question

Using RNA-sequencing analysis, we investigated the relationship between ovarian stimulation and endometrial transcriptome profiles during the window of implantation (WOI) to identify candidate predictive factors for the WOI and to optimize timing for embryo transfer.

Methods

Twelve women with normal basal hormone levels and regular ovulation were randomly assigned into three groups based on sampling time: late-proliferate phase (P group), and receptive phase in natural cycles (LH+7, N group) and stimulated cycles (hCG+7, S group). Transcriptome profiles of mRNAs and long non-coding RNAs (lncRNAs) were then compared among the three groups. Validation was performed using real-time qPCR.

Results

Comparison of transcriptome profiles between the natural a

SUBMITTER: Li L 

PROVIDER: S-EPMC7000608 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature