Quantification of HER1, HER2 and HER3 by time-resolved Forster resonance energy transfer in FFPE triple-negative breast cancer samples.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) has a worse prognosis compared with other breast cancer subtypes, and biomarkers to identify patients at high risk of recurrence are needed. Here, we investigated the expression of human epidermal receptor (HER) family members in TNBC and evaluated their potential as biomarkers of recurrence.

Methods

We developed Time Resolved-Förster Resonance Energy Transfer (TR-FRET) assays to quantify HER1, HER2 and HER3 in formalin-fixed paraffin-embedded (FFPE) tumour tissues. After assessing the performance and precision of our assays, we quantified HER protein expression in 51 TNBC specimens, and investigated the association of their expression with relapse-free survival.

Results

The assays were quantitative, accurate, and robust

SUBMITTER: Ho-Pun-Cheung A 

PROVIDER: S-EPMC7000684 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature