Characterization of Myeloid Cellular Populations in Mesenteric and Subcutaneous Adipose Tissue of Holstein-Friesian Cows.

ABSTRACT: Immune cells resident in adipose tissue have important functions in local and systemic metabolic homeostasis. Nevertheless, these immune cell populations remain poorly characterized in bovines. Recently, we described diverse lymphocyte subpopulations in adipose tissue of Holstein-Friesian cows. Here, we aimed at characterising myeloid cell populations present in bovine adipose tissue using multicolour flow cytometry, cell sorting and histochemistry/immunohistochemistry. Macrophages, CD14⁺CD11b⁺MHC-II⁺CD45⁺ cells, were identified in mesenteric and subcutaneous adipose tissue, though at higher proportions in the latter. Mast cells, identified as SSC-A^highCD11b^-/+CD14^-MHC-II^-CH138A^-CD45⁺

SUBMITTER: Oliveira BM 

PROVIDER: S-EPMC7000716 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature