FKBPL-based peptide, ALM201, targets angiogenesis and cancer stem cells in ovarian cancer.

ABSTRACT:

Background

ALM201 is a therapeutic peptide derived from FKBPL that has previously undergone preclinical and clinical development for oncology indications and has completed a Phase 1a clinical trial in ovarian cancer patients and other advanced solid tumours.

Methods

In vitro, cancer stem cell (CSC) assays in a range of HGSOC cell lines and patient samples, and in vivo tumour initiation, growth delay and limiting dilution assays, were utilised. Mechanisms were determined by using immunohistochemistry, ELISA, qRT-PCR, RNAseq and western blotting. Endogenous FKBPL protein levels were evaluated using tissue microarrays (TMA).

Results

ALM201 reduced CSCs in cell lines and primary samples by inducing differentiation. ALM201 treatment of highly vascularised Kuramochi xenogra

SUBMITTER: Annett S 

PROVIDER: S-EPMC7000737 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature