Stathmin levels alter PTPN14 expression and impact neuroblastoma cell migration.

ABSTRACT:

Background

Stathmin mediates cell migration and invasion in vitro, and metastasis in vivo. To investigate stathmin's role on the metastatic process, we performed integrated mRNA-miRNA expression analysis to identify pathways regulated by stathmin.

Methods

MiRNA and gene arrays followed by miRNA-target-gene integration were performed on stathmin-depleted neuroblastoma cells (Ctrl_shRNA vs. Stmn Seq2_shRNA). The expression of the predicted target PTPN14 was evaluated by RT-qPCR, western blot and immunohistochemistry. Gene-silencing technology was used to assess the role of PTPN14 on proliferation, migration, invasion and signalling pathway.

Results

Stathmin levels modulated the expression of genes and miRNA in neuroblastoma cells, leading to a deregu

SUBMITTER: Po'uha ST 

PROVIDER: S-EPMC7000740 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature