Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip.

ABSTRACT: Fast and accurate detection of viral RNA pathogens is important in apiculture. A polymerase chain reaction (PCR)-based detection method has been developed, which is simple, specific, and sensitive. In this study, we rapidly (in 1 min) synthesized cDNA from the RNA of deformed wing virus (DWV)-infected bees (Apis mellifera), and then, within 10 min, amplified the target cDNA by ultra-rapid qPCR. The PCR products were hybridized to a DNA-chip for confirmation of target gene specificity. The results of this study suggest that our method might be a useful tool for detecting DWV, as well as for the diagnosis of RNA virus-mediated diseases on-site.

SUBMITTER: Kim JM 

PROVIDER: S-EPMC7000893 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature