Hap2-Ino80-facilitated transcription promotes de novo establishment of CENP-A chromatin.

ABSTRACT: Centromeres are maintained epigenetically by the presence of CENP-A, an evolutionarily conserved histone H3 variant, which directs kinetochore assembly and hence centromere function. To identify factors that promote assembly of CENP-A chromatin, we affinity-selected solubilized fission yeast CENP-A^Cnp1 chromatin. All subunits of the Ino80 complex were enriched, including the auxiliary subunit Hap2. Chromatin association of Hap2 is Ies4-dependent. In addition to a role in maintenance of CENP-A^Cnp1 chromatin integrity at endogenous centromeres, Hap2 is required for de novo assembly of CENP-A^Cnp1 chromatin on naïve centromere DNA and promotes H3 turnover on centromere regions and other loci prone to CENP-A^Cnp1 deposition. Prior to CENP-A^Cnp1

SUBMITTER: Singh PP 

PROVIDER: S-EPMC7000912 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature