Congenital cystic adenomatoid malformations of the lung: an epithelial transcriptomic approach.

ABSTRACT:

Background

The pathophysiology of congenital cystic adenomatoid malformations (CCAM) of the lung remains poorly understood.

Aim

This study aimed to identify more precisely the molecular mechanisms limited to a compartment of lung tissue, through a transcriptomic analysis of the epithelium of macrocystic forms.

Methods

Tissue fragments displaying CCAM were obtained during planned surgical resections. Epithelial mRNA was obtained from cystic and normal areas after laser capture microdissection (LCM). Transcriptomic analyses were performed and the results were confirmed by RT-PCR and immunohistochemistry in independent samples.

Results

After controlling for RNA quality, we analysed the transcriptomes of six cystic areas and five control areas. In total, 393 transc

SUBMITTER: Lezmi G 

PROVIDER: S-EPMC7001206 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature