Nanopore sequencing-based genome assembly and evolutionary genomics of circum-basmati rice.

ABSTRACT:

Background

The circum-basmati group of cultivated Asian rice (Oryza sativa) contains many iconic varieties and is widespread in the Indian subcontinent. Despite its economic and cultural importance, a high-quality reference genome is currently lacking, and the group's evolutionary history is not fully resolved. To address these gaps, we use long-read nanopore sequencing and assemble the genomes of two circum-basmati rice varieties.

Results

We generate two high-quality, chromosome-level reference genomes that represent the 12 chromosomes of Oryza. The assemblies show a contig N50 of 6.32 Mb and 10.53 Mb for Basmati 334 and Dom Sufid, respectively. Using our highly contiguous assemblies, we characterize structural variations segregating across circum-basmati genomes. We discov

SUBMITTER: Choi JY 

PROVIDER: S-EPMC7001208 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature