F0F1 ATP synthase regulates extracellular calcium influx in human neutrophils by interacting with Ca_v2.3 and modulates neutrophil accumulation in the lipopolysaccharide-challenged lung.

ABSTRACT:

Background

Neutrophils form the first line of innate host defense against invading microorganisms. We previously showed that F0F1 ATP synthase (F-ATPase), which is widely known as mitochondrial respiratory chain complex V, is expressed in the plasma membrane of human neutrophils and is involved in regulating cell migration. Whether F-ATPase performs cellular functions through other pathways remains unknown.

Methods

Blue native polyacrylamide gel electrophoresis followed by nano-ESI-LC MS/MS identification and bioinformatic analysis were used to identify protein complexes containing F-ATPase. Then, the identified protein complexes containing F-ATPase were verified by immunoblotting, immunofluorescence colocalization, immunoprecipitation, real-time RT-PCR and agarose gel elect

SUBMITTER: Zhu B 

PROVIDER: S-EPMC7001235 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature