Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer.

ABSTRACT:

Background

With the increasing incidence of colorectal cancer (CRC), its accurate diagnosis is critical and in high demand. However, conventional methods are not ideal due to invasiveness and low accuracy. Herein, we aimed to identify efficient CRC mRNA markers in a non-invasive manner using CRC-derived extracellular vesicles (EVs). The expression levels of EV mRNAs from cancer cell lines were compared with those of a normal cell line using quantitative polymerase chain reaction. Eight markers were evaluated in plasma EVs from CRC patients and healthy controls. The diagnostic value of each marker, individually or in combination, was then determined using recessive operating characteristics analyses and the Mann-Whitney U test.

Results

Eight mRNA markers (MYC, VEGF, CDX2, CD1

SUBMITTER: Cha BS 

PROVIDER: S-EPMC7001337 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature