Fluorescence-based method is more accurate than counting-based methods for plotting growth curves of adherent cells.

ABSTRACT:

Objective

Cell growth curves constitute one of the primary assays employed to analyze cell proliferation dynamics of in vitro cultured cells under specific culture conditions. From the cell growth curve, it is possible to assess the behavior of proliferating cells under different conditions, such as drug treatment and genomic editions. Traditionally, growth curves for adherent cells are obtained by seeding the cells in multiple-well plates and counting the total number of cells at different time points. Here, we compare this traditional method to the fluorescence-based method, which is based on the CFSE fluorescence decay over time.

Results

The fluorescence-based method is not dependent on the determination of the total number of cells, but rather is approached by assessing

SUBMITTER: Pereira TF 

PROVIDER: S-EPMC7001368 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature