Dataset Information

Molecular Characterization and Phylogenetic Analysis of Enterocytozoon bieneusi in Lambs in Oromia Special Zone, Central Ethiopia.

ABSTRACT: Enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and occurs in a wide range of animals. This study was conducted in Central Ethiopia to determine the prevalence and genotypes of E. bieneusi in lambs in order to evaluate their public health significance. Three hundred eighty nine fecal samples were collected and screened using a nested PCR targeting the internal transcribed spacer (ITS) of the ribosomal RNA gene. All positive PCR products were sequenced to determine the genotypes. E. bieneusi was found in 39 (10.03%) of the lambs. Differences in the infection rates among sex and age groups were not significant (P > 0.05). Five ITS genotypes belonging to three known genotypes BEB6, COS-I, and COS-II, and two novel genotypes (ET-L1 and ET-L2) were identified in lambs. All five genotypes identified in the present study clustered within cattle-specific Group 2 in the ITS phylogenetic tree. This first molecular detection and characterization of E. bieneusi in lambs in Ethiopia has identified the need for further studies in humans and other domestic animals in order to determine the public health significance of E. bieneusi in Ethiopia.

SUBMITTER: Wegayehu T

PROVIDER: S-EPMC7001644 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Molecular Characterization and Phylogenetic Analysis of Enterocytozoon bieneusi in Lambs in Oromia Special Zone, Central Ethiopia.

Wegayehu Teklu T Li Junqiang J Karim Md Robiul MR Zhang Longxian L

Frontiers in veterinary science 20200129

Enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and occurs in a wide range of animals. This study was conducted in Central Ethiopia to determine the prevalence and genotypes of E. bieneusi in lambs in order to evaluate their public health significance. Three hundred eighty nine fecal samples were collected and screened using a nested PCR targeting the internal transcribed spacer (ITS) of the ribosomal RNA gene. All positive PCR products wer ...[more]

PMID: 32083097

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Project description:BackgroundCoronavirus disease 2019 (COVID-19) is a highly contagious viral infection, and it has negative effects on public health. The practice of preventive measures of the disease supports containment processes of the spread of coronavirus. However, the practice of preventive measures is affected by several associated risk factors.ObjectiveThis study aimed to investigate the associated factors that limit the youths' practice of preventive measures against COVID-19 in the study area.MethodsA community-based cross-sectional study was conducted. The study used a quantitative approach for collecting data from 384 youths using a survey method. Not practicing preventive measures was measured to determine whether or not youths applied hygiene practices, kept their distance, restricted their movements, and sought self-help or support in the past two months. Descriptive statistics were used to assess the distribution of study participants, and a binary regression model was executed to examine the association factors with inability to practice preventive measures with a p-value < 0.05 statistically significant.ResultsMale youths (Adjusted Odds Ratio (AOR) = 0.06; 95% CI: 0.02, 0.16) were less likely to practice preventive measures. Older youth (AOR = 1.33; 95% CI: 1.13, 1.56), with higher education level (AOR = 1.03; 95% CI: 1.01, 1.06), and who had higher income (AOR = 1.34; 95% CI: 1.02, 1.78) were more likely to practice preventive measures. Further, the belief in the body's immunity to resist the disease (AOR = 0.27; 95% CI: 0.11, 0.67), lack of paying attention to the disease (AOR = 0.07; 95% CI: 0.01, 0.73), ignorance of evidence to the disease (AOR = 0.31; 95% CI: 0.13, 0.74), ease of restriction of movements (AOR = 0.29; 95% CI: 0.12, 0.72), lack of sensitization to actions in the community (AOR = 0.39; 95% CI: 0.16, 0.96), and substance use (AOR = 0.11; 95% CI: 0.05, 0.21) were other factors that were inversely related to practicing preventive measures.ConclusionsThe findings suggested that more intervention efforts, by either communicating to or reaching out all groups, should be employed. All segments of the population should be equipped with the facts that effectively support them practice preventive measures against the disease. Finally, the results suggested that youths should abstain from substance use, keep their distance in their pastime and avoid crowdings.

Dataset Information

Molecular Characterization and Phylogenetic Analysis of Enterocytozoon bieneusi in Lambs in Oromia Special Zone, Central Ethiopia.

Publications

Molecular Characterization and Phylogenetic Analysis of <i>Enterocytozoon bieneusi</i> in Lambs in Oromia Special Zone, Central Ethiopia.

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