Large-Scale, Quantitative Protein Assays on a High-Throughput DNA Sequencing Chip.

ABSTRACT: High-throughput DNA sequencing techniques have enabled diverse approaches for linking DNA sequence to biochemical function. In contrast, assays of protein function have substantial limitations in terms of throughput, automation, and widespread availability. We have adapted an Illumina high-throughput sequencing chip to display an immense diversity of ribosomally translated proteins and peptides and then carried out fluorescence-based functional assays directly on this flow cell, demonstrating that a single, widely available high-throughput platform can perform both sequencing-by-synthesis and protein assays. We quantified the binding of the M2 anti-FLAG antibody to a library of 1.3 × 10⁴ variant FLAG peptides, exploring non-additive effects of combinations of mutations and disco

SUBMITTER: Layton CJ 

PROVIDER: S-EPMC7001660 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature