Multiplex chromatin interactions with single-molecule precision.

ABSTRACT: The genomes of multicellular organisms are extensively folded into 3D chromosome territories within the nucleus¹. Advanced 3D genome-mapping methods that combine proximity ligation and high-throughput sequencing (such as chromosome conformation capture, Hi-C)², and chromatin immunoprecipitation techniques (such as chromatin interaction analysis by paired-end tag sequencing, ChIA-PET)³, have revealed topologically associating domains⁴ with frequent chromatin contacts, and have identified chromatin loops mediated by specific protein factors for insulation and regulation of transcription^5-7. However, these methods rely on pairwise proximity ligation and reflect population-level views, and thus cannot reveal the detailed nature of chromati

SUBMITTER: Zheng M 

PROVIDER: S-EPMC7001875 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature