Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera.

ABSTRACT: Wood and wood products can harbor microorganisms that can raise phytosanitary concerns in countries importing or exporting these products. To evaluate the efficacy of wood treatment on the survival of microorganisms of phytosanitary concern the method of choice is to grow microbes in petri dishes for subsequent identification. However, some plant pathogens are difficult or impossible to grow in axenic cultures. A molecular methodology capable of detecting living fungi and fungus-like organisms in situ can provide a solution. RNA represents the transcription of genes and can become rapidly unstable after cell death, providing a proxy measure of viability. We designed and used RNA-based molecular diagnostic assays targeting genes essential to vital processes and assessed their presence in wo

SUBMITTER: Wong B 

PROVIDER: S-EPMC7001964 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature