3D mapping of native extracellular matrix reveals cellular responses to the microenvironment.

ABSTRACT: Cells and extracellular matrix (ECM) are mutually interdependent: cells guide self-assembly of ECM precursors, and the resulting ECM architecture supports and instructs cells. Though bidirectional signaling between ECM and cells is fundamental to cell biology, it is challenging to gain high-resolution structural information on cellular responses to the matrix microenvironment. Here we used cryo-scanning transmission electron tomography (CSTET) to reveal the nanometer- to micron-scale organization of major fibroblast ECM components in a native-like context, while simultaneously visualizing internal cell ultrastructure including organelles and cytoskeleton. In addition to extending current models for collagen VI fibril organization, three-dimensional views of thick cell regions and surroundi

SUBMITTER: Lansky Z 

PROVIDER: S-EPMC7001979 | biostudies-literature | 2019 Jan-Mar

REPOSITORIES: biostudies-literature