Area and Geometry Dependence of Cell Migration in Asymmetric Two-State Micropatterns.

ABSTRACT: Microstructured surfaces provide a unique framework to probe cell migration and cytoskeletal dynamics in a standardized manner. Here, we report on the steady-state occupancy probability of cells in asymmetric two-state microstructures that consist of two fibronectin-coated adhesion sites connected by a thin guidance cue. In these dumbbell-like structures, cells transition between the two sites in a repeated and stochastic manner, and average dwell times in the respective microenvironments are determined from the cell trajectories. We study the dynamics of human breast carcinoma cells (MDA-MB-231) in these microstructures as a function of area, shape, and orientation of the adhesion sites. On square adhesive sites with different areas, we find that the occupancy probability ratio is directl

SUBMITTER: Fink A 

PROVIDER: S-EPMC7002917 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature