Inflammation-Induced Alternative Pre-mRNA Splicing in Mouse Alveolar Macrophages.

ABSTRACT: Alveolar macrophages serve as central orchestrators of inflammatory responses in the lungs, both initiating their onset and promoting their resolution. However, the mechanisms that program macrophages for these dynamic responses are not fully understood. Over 95% of all mammalian genes undergo alternative pre-mRNA splicing. While alternative splicing has been shown to regulate inflammatory responses in macrophages in vitro, it has not been investigated on a genome-wide scale in vivo Here we used RNAseq to investigate alternative pre-mRNA splicing in alveolar macrophages isolated from lipopolysaccharide (LPS)-treated mice during the peak of inflammation and during its resolution. We found that lung inflammation induced substantial alternative pre-mRNA splicing in alveolar macrophages. The n

SUBMITTER: Janssen WJ 

PROVIDER: S-EPMC7003074 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature