Identification of the Genetic Requirements for Zinc Tolerance and Toxicity in Saccharomyces cerevisiae.

ABSTRACT: Zinc is essential for almost all living organisms, since it serves as a crucial cofactor for transcription factors and enzymes. However, it is toxic to cell growth when present in excess. The present work aims to investigate the toxicity mechanisms induced by zinc stress in yeast cells. To this end, 108 yeast single-gene deletion mutants were identified sensitive to 6 mM ZnCl2 through a genome-wide screen. These genes were predominantly related to the biological processes of vacuolar acidification and transport, polyphosphate metabolic process, cytosolic transport, the process utilizing autophagic mechanism. A result from the measurement of intracellular zinc content showed that 64 mutants accumulated higher intracellular zinc under zinc stress than the wild-type cells. We further measured

SUBMITTER: Zhao YY 

PROVIDER: S-EPMC7003084 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature