Identification of Urinary Biomarkers for Exercise-Induced Immunosuppression by iTRAQ Proteomics.

ABSTRACT:

Purpose

To identify noninvasive immune biomarkers of exercise-induced immunosuppression using the iTRAQ proteomics technique.

Methods

Fifteen healthy males were recruited and subjected to a four-week incremental treadmill running training program. After each week of training, WBC counts and CD4⁺ and CD8⁺ lymphocytes were measured to monitor the immune function status. iTRAQ proteomics technology was used to identify differential proteins and their characteristics in urine.

Results

Our data showed that the WBC counts, CD4⁺ lymphocytes, and CD4⁺/CD8⁺ ratio decreased by more than 10% after four weeks of training, suggesting exercise-induced immunosuppression. A total of 1854 proteins were identified in urine during t

SUBMITTER: Xu G 

PROVIDER: S-EPMC7003279 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature