Identification of a Yarrowia lipolytica acetamidase and its use as a yeast genetic marker.

ABSTRACT: BACKGROUND:Yarrowia lipolytica is an oleaginous yeast that can be genetically engineered to produce lipid and non-lipid biochemicals from a variety of feedstocks. Metabolic engineering of this organism usually requires genetic markers in order to select for modified cells. The potential to combine multiple genetic manipulations depends on the availability of multiple or recyclable selectable markers. RESULTS:We found that Y. lipolytica has the ability to utilize acetamide as the sole nitrogen source suggesting that the genome contains an acetamidase gene. Two potential Y. lipolytica acetamidase gene candidates were identified by homology to the A. nidulans acetamidase amdS. These genes were deleted in the wild-type Y. lipolytica strain YB-392, and deletion strains were evaluated for acetam

SUBMITTER: Hamilton M 

PROVIDER: S-EPMC7003347 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature