?-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines.

ABSTRACT: Background:Cholangiocarcinoma (CCA) is a neglected disease prevalent in developing countries with high burden and mortality rate, and there is no effective treatment. We aimed to investigate ?-eudesmol molecular target of action in human CCA cell lines using the selected key molecules of apoptotic pathways. Materials and Methods:Two CCA cell lines (HuH28 and HuCCT1) were assessed at different time points after ?-eudesmol treatment for mRNA and protein expression profiles of caspase-3, -8, -9, p53, p21, Bcl-2, and Bax by real-time polymerase chain reaction and western blot, respectively. Results:?-eudesmol induced expressions of p21 and p53 in mRNA/protein level in HuH28 and HuCCT1 cells. These CCA cells also expressed caspase-3, -8, -9 and bax (mRNA and/or protein level) among others after ?-eudesmol treatment indicating its role in both intrinsic and extrinsic caspase-dependent apoptotic pathways. Conclusion:The study demonstrated that ?-eudesmol induced the expression of apoptosis pathway proteins, suggesting its potential role in promoting the caspase-dependent apoptotic pathway, and induction of the cell cycle arrest in CCA cell lines. ?-eudesmol can be considered as a potential compound for further investigation as an anti-CCA agent.

SUBMITTER: Narahara C 

PROVIDER: S-EPMC7003544 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature