LrrkA, a kinase with leucine-rich repeats, links folate sensing with Kil2 activity and intracellular killing.

ABSTRACT: Phagocytic cells ingest bacteria by phagocytosis and kill them efficiently inside phagolysosomes. The molecular mechanisms involved in intracellular killing and their regulation are complex and still incompletely understood. Dictyostelium discoideum has been used as a model to discover and to study new gene products involved in intracellular killing of ingested bacteria. In this study, we performed random mutagenesis of Dictyostelium cells and isolated a mutant defective for growth on bacteria. This mutant is characterized by the genetic inactivation of the lrrkA gene, which encodes a protein with a kinase domain and leucine-rich repeats. LrrkA knockout (KO) cells kill ingested Klebsiella pneumoniae bacteria inefficiently. This defect is not additive to the killing defect observed in kil2

SUBMITTER: Bodinier R 

PROVIDER: S-EPMC7003747 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature