Practical Synthesis of Cap-4 RNA.

ABSTRACT: Eukaryotic mRNAs possess 5' caps that are determinants for their function. A structural characteristic of 5' caps is methylation, with this feature already present in early eukaryotes such as Trypanosoma. While the common cap-0 (m⁷ GpppN) shows a rather simple methylation pattern, the Trypanosoma cap-4 displays seven distinguished additional methylations within the first four nucleotides. The study of essential biological functions mediated by these unique structural features of the cap-4 and thereby of the metabolism of an important class of human pathogenic parasites is hindered by the lack of reliable preparation methods. Herein we describe the synthesis of custom-made nucleoside phosphoramidite building blocks for m⁶ ₂ Am and m³ Um, their inc

SUBMITTER: Leiter J 

PROVIDER: S-EPMC7004131 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature