A shear stress micromodel of urinary tract infection by the Escherichia coli producing Dr adhesin.

ABSTRACT: In this study, we established a dynamic micromodel of urinary tract infection to analyze the impact of UT-segment-specific urinary outflow on the persistence of E. coli colonization. We found that the adherence of Dr+ E. coli to bladder T24 transitional cells and type IV collagen is maximal at lowest shear stress and is reduced by any increase in flow velocity. The analyzed adherence was effective in the whole spectrum of physiological shear stress and was almost irreversible over the entire range of generated shear force. Once Dr+ E. coli bound to host cells or collagen, they did not detach even in the presence of elevated shear stress or of chloramphenicol, a competitive inhibitor of binding. Investigating the role of epithelial surface architecture, we showed that the presence of buddin

SUBMITTER: Zalewska-Piatek B 

PROVIDER: S-EPMC7004390 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature