Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters.

ABSTRACT: Bidirectional green tissue-specific promoters have important application prospects in genetic engineering and crop genetic improvement. However, there is no report on the application of them, mainly due to undiscovered natural bidirectional green tissue-specific promoters and the lack of a comprehensive approach for the synthesis of these promoters. In order to compensate for this vacancy, the present study reports a novel strategy for the expression regulatory sequence selection and the bidirectional green tissue-specific synthetic promoter construction. Based on this strategy, seven promoters were synthesized and introduced into rice by agrobacterium-mediated transformation. The functional identification of these synthetic promoters was performed by the expression pattern of GFP and GUS

SUBMITTER: Bai J 

PROVIDER: S-EPMC7004895 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature