DREB/CBF expression in wheat and barley using the stress-inducible promoters of HD-Zip I genes: impact on plant development, stress tolerance and yield.

ABSTRACT: Networks of transcription factors regulate diverse physiological processes in plants to ensure that plants respond to abiotic stresses rapidly and efficiently. In this study, expression of two DREB/CBF genes, TaDREB3 and TaCBF5L, was modulated in transgenic wheat and barley, by using stress-responsive promoters HDZI-3 and HDZI-4. The promoters were derived from the durum wheat genes encoding the γ-clade TFs of the HD-Zip class I subfamily. The activities of tested promoters were induced by drought and cold in leaves of both transgenic species. Differences in sensitivity of promoters to drought strength were dependent on drought tolerance levels of cultivars used for generation of transgenic lines. Expression of the DREB/CBF genes under both promoters improved drought and frost tolerance of

SUBMITTER: Yang Y 

PROVIDER: S-EPMC7004899 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature