Developing a xenograft model of human vasculature in the mouse ear pinna.

ABSTRACT: Humanised xenograft models allow for the analysis of human tissue within a physiological environment in vivo. However, current models often rely on the angiogenesis and ingrowth of recipient vasculature to perfuse tissues, preventing analysis of biological processes and diseases involving human blood vessels. This limits the effectiveness of xenografts in replicating human physiology and may lead to issues with translating findings into human research. We have designed a xenograft model of human vasculature to address this issue. Human subcutaneous fat was cultured in vitro to promote blood vessel outgrowth prior to implantation into immunocompromised mice. We demonstrate that implants survived, retained human vasculature and anastomosed with the circulatory system of the recipient mouse.

SUBMITTER: Meehan GR 

PROVIDER: S-EPMC7004987 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature