Robust RNA-Seq of aRNA-amplified single cell material collected by patch clamp.

ABSTRACT: Most single cell RNA sequencing protocols start with single cells dispersed from intact tissue. High-throughput processing of the separated cells is enabled using microfluidics platforms. However, dissociation of tissue results in loss of information about cell location and morphology and potentially alters the transcriptome. An alternative approach for collecting RNA from single cells is to re-purpose the electrophysiological technique of patch clamp recording. A hollow patch pipette is attached to individual cells, enabling the recording of electrical activity, after which the cytoplasm may be extracted for single cell RNA-Seq ("Patch-Seq"). Since the tissue is not disaggregated, the location of cells is readily determined, and the morphology of the cells is maintained, making possible t

SUBMITTER: Kim JMH 

PROVIDER: S-EPMC7004989 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature