Development of a real-time PCR assay for the identification and quantification of bovine ingredient in processed meat products.

ABSTRACT: In order to find fraudulent species substitution in meat products, a highly sensitive and rapid assay for meat species identification and quantification is urgently needed. In this study, species-specific primers and probes were designed from the mitochondrial cytb (cytochrome b) fragment for identification and quantification of bovine ingredient in commercial meat products. Bovine samples and non-bovine ones were used to identify the specificity, sensitivity, and applicability of established assay. Results showed that the primers and probes were highly specific for bovine ingredient in meat products. The absolute detection limit of the real-time PCR method was 0.025 ng DNA, and the relative detection limit was 0.002% (w/w) of positive samples. The quantitative real-time PCR assay was vali

SUBMITTER: Chen X 

PROVIDER: S-EPMC7004997 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature