Formate dehydrogenase, ubiquinone, and cytochrome bd-I are required for peptidoglycan recognition protein-induced oxidative stress and killing in Escherichia coli.

ABSTRACT: Mammalian Peptidoglycan Recognition Proteins (PGRPs) kill bacteria through induction of synergistic oxidative, thiol, and metal stress. PGRPs induce oxidative stress in bacteria through a block in the respiratory chain, which results in decreased respiration and incomplete reduction of oxygen (O₂) to hydrogen peroxide (H₂O₂). In this study we identify the site of PGRP-induced generation of H₂O₂ in Escherichia coli. Tn-seq screening of E. coli Tn10 insertion library revealed that mutants in formate dehydrogenase (FDH) genes had the highest survival following PGRP treatment. Mutants lacking functional FDH-O had abolished PGRP-induced H₂O₂ production and the highest resistance to PGRP-induced killing, and formate enh

SUBMITTER: Kashyap DR 

PROVIDER: S-EPMC7005000 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature