Automated high-throughput heartbeat quantification in medaka and zebrafish embryos under physiological conditions.

ABSTRACT: Accurate quantification of heartbeats in fish models is an important readout to study cardiovascular biology, disease states and pharmacology. However, dependence on anaesthesia, laborious sample orientation or requirement for fluorescent reporters have hampered the use of high-throughput heartbeat analysis. To overcome these limitations, we established an efficient screening assay employing automated label-free heart rate determination of randomly oriented, non-anesthetized medaka (Oryzias latipes) and zebrafish (Danio rerio) embryos in microtiter plates. Automatically acquired bright-field data feeds into an easy-to-use HeartBeat software with graphical user interface for automated quantification of heart rate and rhythm. Sensitivity of the assay was demonstrated by profiling heart rates

SUBMITTER: Gierten J 

PROVIDER: S-EPMC7005164 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature