A rare subpopulation of melanoma cells with low expression of metastasis suppressor NME1 is highly metastatic in vivo.

ABSTRACT: Despite recent advances in melanoma treatment, metastasis and resistance to therapy remain serious clinical challenges. NME1 is a metastasis suppressor, a class of proteins which inhibits metastatic spread of cancer cells without impact on growth of the primary tumor. We have identified a rare subpopulation of cells with markedly reduced expression of NME1 (NME1^LOW) in human melanoma cell lines. To enable isolation of viable NME1^LOW cells for phenotypic analysis by fluorescence-activated cell sorting (FACS), a CRISPR-Cas9-mediated approach was used to attach an EGFP coding module to the C-terminus of the endogenous NME1 gene in melanoma cell lines. NME1^LOW cells displayed enhanced collective invasion in vitro when implanted as 3D aggregates in Matrigel. NME

SUBMITTER: Snyder D 

PROVIDER: S-EPMC7005181 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature