Thickness-wise growth technique for human articular chondrocytes to fabricate three-dimensional cartilage grafts.

ABSTRACT:

Introduction

Cutting the cost of manufacturing is important for extending the use of tissue-engineered therapeutic products. The present study aimed to develop a simple method for fabrication of cartilaginous tissues for regenerative therapy, utilizing the phenomenon where human articular chondrocytes grow thickness-wise and spontaneously form three-dimensionally thick tissues.

Methods

Normal human articular chondrocytes (NHACs) were cultured with varying concentrations of transforming growth factor beta 1 (TGF-β1) and/or fibroblast growth factor-2 (FGF-2) to optimize the culture condition for thickness-wise growth of chondrocytes. Next, the tissues grown in the optimal condition were subjected to re-differentiation culture in attached and detached states to assess different

SUBMITTER: Kikuchi T 

PROVIDER: S-EPMC7005340 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature