Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens.

ABSTRACT: The aim of this study was to examine whether Salmonella enteritidis (SE) vaccination affects innate immune function and histone modifications responsible for epigenetic reprogramming in the follicular theca of laying hens. White Leghorn laying hens were administered the SE vaccine or phosphate buffered saline (PBS; control) one week before sample collection. The largest follicles (F1) were collected for total RNA and histone protein extraction. Gene expression levels of immune molecules (Toll-like receptors [TLRs], cytokines, and avian β-defensins [AvBDs]), and histone modifications in the follicular thecal tissues, were examined using real-time PCR and western blot, respectively. The results showed that the expression levels of TLR1-1, 2-1, 4, and 15 were upregulated by SE vaccination. Al

SUBMITTER: Kang Y 

PROVIDER: S-EPMC7005392 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature