Clip-Domain Serine Protease Gene (LsCLIP3) Is Essential for Larval-Pupal Molting and Immunity in Lasioderma serricorne.

ABSTRACT: Clip-domain serine proteases (CLIPs) play crucial roles in insect development and innate immunity. In this study, we identified a CLIP gene (designated LsCLIP3) from the cigarette beetle Lasioderma serricorne. LsCLIP3 contains a 1,773-bp open reading frame (ORF) encoding a 390-amino-acid protein and shows a conserved clip domain and a trypsin-like serine protease domain. Phylogenetic analysis indicated that LsCLIP3 was orthologous to the CLIP-B subfamily. LsCLIP3 was prominently expressed in larva, pupa, and early adult stages. In larval tissues, it was highly expressed in the integument and fat body. The expression of LsCLIP3 was induced by 20-hydroxyecdysone. A similar induction was also found by peptidoglycans from Escherichia coli and

SUBMITTER: Yang WJ 

PROVIDER: S-EPMC7005593 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature