Circ_0000267 promotes gastric cancer progression via sponging MiR-503-5p and regulating HMGA2 expression.

ABSTRACT:

Background

Circular RNAs (circRNAs) are a class of newly discovered RNAs that attach great importance to modulate gene expression and biological function. Nonetheless, in gastric cancer (GC), the expression and function of circRNA are much less explored. In this study, circ_0000267 expression in GC was investigated and the function and mechanism of circ_0000267 was probed.

Materials and methods

Quantitative real-time PCR (qRT-PCR) was employed to detect circ_0000267, miR-503-5p, and HMGA2 expression. Immunohistochemistry and western blot were adopted to detect HMGA2 and epithelial-mesenchymal transition (EMT)-related proteins (E-cadherin and N-cadherin) expression in GC tissues and cells, respectively. GC cell lines with circ_0000267 overexpressed and knocked down were const

SUBMITTER: Cai X 

PROVIDER: S-EPMC7005624 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature