IGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche.

ABSTRACT: Our understanding of the signalling pathways regulating early human development is limited, despite their fundamental biological importance. Here, we mine transcriptomics datasets to investigate signalling in the human embryo and identify expression for the insulin and insulin growth factor 1 (IGF1) receptors, along with IGF1 ligand. Consequently, we generate a minimal chemically-defined culture medium in which IGF1 together with Activin maintain self-renewal in the absence of fibroblast growth factor (FGF) signalling. Under these conditions, we derive several pluripotent stem cell lines that express pluripotency-associated genes, retain high viability and a normal karyotype, and can be genetically modified or differentiated into multiple cell lineages. We also identify active phosphoinosi

SUBMITTER: Wamaitha SE 

PROVIDER: S-EPMC7005693 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature